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KMID : 1161420110140121538
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Journal of Medicinal Food
2011 Volume.14 No. 12 p.1538 ~ p.1545
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The Effect of Crocus sativus Extract on Human Lymphocytes' Cytokines and T Helper 2/T Helper 1 Balance
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Boskabady Mohammad Hossein
Tamijani Seyedh Masoumeh Seyedhosseini
Rafatpanah Houshang
Rezaei Abadolrahim
Alavinejad Azam
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Abstract
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The effects of macerated extracts of Crocus sativus (Family Iridaceae) (saffron) on cell viability and cytokine release of stimulated peripheral blood mononuclear cells by phytohemagglutinin (PHA) and nonstimulated cells were examined. The effects of three concentrations of macerated extract, dexamethasone, and saline on cell viability and production of cytokines, including interleukin (IL)-4, IL-10, and interferon-γ (IFN-γ) were evaluated. In cells stimulated with PHA, different concentrations of the extract significantly inhibited cell viability of lymphocytes (P<.001 for all concentrations). High concentrations of the extract (500?μg/mL) also inhibited secretion of IFN-γ in stimulated cells and IL-10 secretion in both stimulated and nonstimulated cells (P<.05 for all cases). The effects of high and low concentrations of the extract (500 and 50?μg/mL, respectively) on IL-4 secretion were lower than that of dexamethasone (P<.05 to P<.001). The extract showed a stimulatory effect on IFN-γ and IL-4 secretion in nonstimulated cells. The ratios of IFN-γ to IL-4 in the presence of all concentrations of saffron on stimulated cells were significantly higher than for the control group (P<.05 to P<.01). These results indicated that the extract of saffron leads to increased ratio of IFN-γ to IL-4.
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KEYWORD
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cell viability, Crocus sativus, cytokine, human lymphocyte, Iridaceae, peripheral mononuclear cell
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